Frataxin Recombinant Rabbit Monoclonal Antibody [PSH15-18]
cat.: HA751547
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH15-18 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Frataxin aa 42-210. |
| Positive control: | SW480 cell lysate, HCT 116 cell lysate, TF-1 cell lysate, Huh7 cell lysate, PC-12 cell lysate, human kidney tissue, human liver tissue. |
| Subcellular location: | Mitochondrion; Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IHC-P |
1:2,000-1:5,000 1:1,000 |
| Uniprot #: | SwissProt: Q16595 Human | O35943 Mouse | D3ZYW7 Rat |
| Alternative names: | CyaY d-FXN FA FARR Frataxin mature form Frataxin(81-210) FRDA FRDA_HUMAN Friedreich ataxia protein Fxn i-FXN m56-FXN m78-FXN m81-FXN MGC57199 X25 |
Images
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Fig1:
Western blot analysis of Frataxin on different lysates with Rabbit anti-Frataxin antibody (HA751547) at 1/5,000 dilution. Lane 1: SW480 cell lysate (20 µg/Lane) Lane 2: HCT 116 cell lysate (20 µg/Lane) Lane 3: TF-1 cell lysate (20 µg/Lane) Lane 4: Huh7 cell lysate (20 µg/Lane) Lane 5: PC-12 cell lysate (20 µg/Lane) Predicted band size: 23 kDa Observed band size: 14/19 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751547) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Frataxin antibody (HA751547) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751547) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Frataxin antibody (HA751547) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751547) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.