KAT3B / p300 Recombinant Rabbit Monoclonal Antibody [PSH15-31]
cat.: HA751553
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH15-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 264 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human KAT3B / p300 aa 51-350. |
| Positive control: | 293T cell lysate, HeLa cell lysate, Jurkat cell lysate, LoVo cell lysate, C2C12 cell lysate, PC-12 cell lysate, COS-1 cell lysate, 293T. |
| Subcellular location: | Cytoplasm, Nucleus, Chromosome. |
| Recommended Dilutions:
WB IF-Cell |
1:5,000 1:100 |
| Uniprot #: | SwissProt: Q09472 Human | B2RWS6 Mouse Entrez Gene: 170915 Rat |
| Alternative names: | E1A associated protein p300 E1A binding protein p300 E1A-associated protein p300 EP300 EP300: E1A binding protein p300 EP300_HUMAN Histone acetyltransferase p300 KAT3B p300 HAT RSTS2 |
Images
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Fig1:
Western blot analysis of KAT3B / p300 on different lysates with Rabbit anti-KAT3B / p300 antibody (HA751553) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: LoVo cell lysate Lane 5: C2C12 cell lysate Lane 6: PC-12 cell lysate Lane 7: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 264 kDa Observed band size: 300 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751553) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of 293T cells labeling KAT3B / p300 with Rabbit anti-KAT3B / p300 antibody (HA751553) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KAT3B / p300 antibody (HA751553) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.