Beta III Tubulin Recombinant Rabbit Monoclonal Antibody [PSH15-32]
cat.: HA751554
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue, IF-Cell, IP, IHC-Fr, FC(Intra) |
| Clonality: | Monoclonal |
| Clone number: | PSH15-32 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 0.1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse Beta III Tubulin aa 401-450. |
| Positive control: | SH-SY5Y cell lysate, U-87 MG cell lysate, Neuro-2a cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, mouse neuron cells, human cerebellum tissue, mouse E14.5 embryo tissue, mouse cerebellum tissue, rat E14.5 embryo tissue, rat cerebellum tissue, SH-SY5Y, Neuro-2a. |
| Subcellular location: | Cytoplasm, cytoskeleton, growth cone, lamellipodium, filopodium. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IF-Cell IP IHC-Fr FC(Intra) |
1:5,000 1:2,000-1:4,000 1:500-1:2,000 1:500-1:1,000 1-2μg/sample 1:500 1:1,000-1:10,000 |
| Uniprot #: | SwissProt: Q13509 Human | Q9ERD7 Mouse | Q4QRB4 Rat |
| Alternative names: | beta 3 tubulin beta 4 beta-4 CDCBM CDCBM1 CFEOM3 CFEOM3A FEOM3 M(beta)3 M(beta)6 MC1R Neuron specific beta III Tubulin Neuron-specific class III beta-tubulin QccE-11995 QccE-15186 TBB3_HUMAN Tubb 3 TUBB3 TUBB4 Tubulin beta 3 Tubulin beta 3 chain Tubulin beta 4 Tubulin beta III Tubulin beta-3 chain Tubulin beta-4 chain Tubulin beta-III tuj 1 tuj1 |
Images
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Fig1:
Application: IF-Tissue Species: Mouse Site: cerebellum Sample: Paraffin-embedded section Antibody concentration: 1/500 |
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Fig2:
Application: IF-Tissue Species: Rat Site: cerebellum Sample: Paraffin-embedded section Antibody concentration: 1/500 |
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Fig3:
Immunocytochemistry analysis of mouse neuron cells labeling Beta III Tubulin with Rabbit anti-Beta III Tubulin antibody (HA751554) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Beta III Tubulin antibody (HA751554) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-Beta III Tubulin antibody (HA751554) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751554) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Beta III Tubulin antibody (HA751554) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751554) at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Beta III Tubulin antibody (HA751554) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751554) at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunocytochemistry analysis of SH-SY5Y (positive) and HeLa (negative) labeling Beta III Tubulin with Rabbit anti-Beta III Tubulin antibody (HA751554) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Beta III Tubulin antibody (HA751554) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig8:
Flow cytometric analysis of HeLa (left, negative) and SH-SY5Y (right, positive) cells labeling Beta III Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751554, 1/10,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig9:
Flow cytometric analysis of Neuro-2a cells labeling Beta III Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751554, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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