p16INK4a Recombinant Rabbit Monoclonal Antibody [PSH15-40]
cat.: HA751555
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH15-40 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Positive control: | A20 cell lysate, MEF cell lysate, mouse spleen tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P IP |
1:2,000 1:200 1:1,000 |
| Uniprot #: | SwissProt: P51480 Mouse |
| Alternative names: | CCM2 CDK4 inhibitor p16 INK4 CDK4I CDKN2 CDKN2A Cell cycle negative regulator beta CMM2 Cyclin dependent kinase 4 inhibitor A Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) Cyclin Dependent Kinase Inhibitor 2A Cyclin dependent kinase inhibitor 2A isoform 4 Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 Cyclin dependent kinase inhibitor p16 INK4 INK4A MLM MTS1 Multiple tumor suppressor 1 p14 p16 P16INK4 p16INK4a p19 p19Arf TP16 |
Images
|
Fig1:
Western blot analysis of p16INK4a on different lysates with Rabbit anti-p16INK4a antibody (HA751555) at 1/2,000 dilution. Lane 1: A20 cell lysate Lane 2: NIH/3T3 cell lysate (negative) Lane 3: MEF cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 16 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751555) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-p16INK4a antibody (HA751555) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751555) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
p16INK4a was immunoprecipitated from 0.2 mg MEF cell lysate with HA751555 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751555 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: MEF cell lysate (input) Lane 2: HA751555 IP in MEF cell lysate Lane 3: Rabbit IgG instead of HA751555 in MEF cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 minutes; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.