WWOX Recombinant Rabbit Monoclonal Antibody [PSH15-43]
cat.: HA751558
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH15-43 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human WWOX aa 1-414. |
| Positive control: | MCF7 cell lysate, HepG2 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, Rat brain tissue lysate, NIH/3T3. |
| Subcellular location: | Cytoplasm, Nucleus, Mitochondrion, Golgi apparatus, Lysosome. |
| Recommended Dilutions:
WB IF-Cell IP |
1:10,000 1:100 1-2μg/sample |
| Uniprot #: | SwissProt: Q9NZC7 Human | Q91WL8 Mouse Entrez Gene: 292041 Rat |
| Alternative names: | 5330426P09Rik 9030416C10Rik Aberrant WW domain-containing oxidoreductase D16S432E EC 1.1.1.- EIEE28 FOR FRA16D Fragile site FRA16D oxidoreductase Fragile site FRA16D Oxireductase HHCMA56 MGC55975 PRO0128 Putative oxidoreductase SCAR12 SDR41C1 Short chain dehydrogenase/reductase family 41C, member 1 WOX1 WW domain containing oxidoreductase WW domain-containing oxidoreductase WW domain-containing protein WWOX wwox WWOX_HUMAN zgc:55975 |
Images
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Fig1:
Western blot analysis of WWOX on different lysates with Rabbit anti-WWOX antibody (HA751558) at 1/10,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: C6 cell lysate (20 µg/Lane) Lane 6: COS-1 cell lysate (20 µg/Lane) Lane 7: Rat brain tissue lysate (30 µg/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751558) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of NIH/3T3 cells labeling WWOX with Rabbit anti-WWOX antibody (HA751558) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-WWOX antibody (HA751558) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
WWOX was immunoprecipitated from 0.2 mg MCF7 cell lysate with HA751558 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751558 at 1/10,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: MCF7 cell lysate (input) Lane 2: HA751558 IP in MCF7 cell lysate Lane 3: Rabbit IgG instead of HA751558 in MCF7 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.