TFPI2 Recombinant Rabbit Monoclonal Antibody [PSH15-45]
cat.: HA751560
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH15-45 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TFPI2 aa 1-235. |
| Positive control: | U-87 MG cell lysate, human placenta tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P IP |
1:5,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P48307 Human |
| Alternative names: | Placental protein 5 PP5 REF1 Retinal pigment epithelium cell factor 1 TFPI 2 TFPI-2 TFPI2 TFPI2 PEN TFPI2_HUMAN Tissue factor pathway inhibitor 2 |
Images
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Fig1:
Western blot analysis of TFPI2 on different lysates with Rabbit anti-TFPI2 antibody (HA751560) at 1/5,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: MCF7 cell lysate (low expression) Lysates/proteins at 20 µg/Lane. Predicted band size: 27 kDa Observed band size: 30 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751560) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of TFPI2 on different lysates with Rabbit anti-TFPI2 antibody (HA751560) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: U-87 MG cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 27 kDa Observed band size: 27/30 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751560) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-TFPI2 antibody (HA751560) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751560) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
TFPI2 was immunoprecipitated from 0.2 mg U-87 MG cell lysate with HA751560 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751560 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: U-87 MG cell lysate (input) Lane 2: HA751560 IP in U-87 MG cell lysate Lane 3: Rabbit IgG instead of HA751560 in U-87 MG cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 minutes; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.