MAGED2 Recombinant Rabbit Monoclonal Antibody [PSH15-53]
cat.: HA751566
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: PSH15-53
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 65 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human MAGED2 aa 151-200.
Positive control: HEK-293 cell lysate, A431 cell lysate, A549 cell lysate, U-2 OS cell lysate.
Subcellular location: Cytosol, extracellular region, membrane, nucleolus, nucleoplasm, nucleus, platelet alpha granule lumen.
Recommended Dilutions:
  WB
1:1,000
Uniprot #: SwissProt: Q9UNF1 Human
Alternative names: 11B6 BCG 1 BCG-1 Breast cancer-associated gene 1 protein HCA10 Hepatocellular carcinoma-associated protein JCL-1 JCL 1 MAGD2_HUMAN MAGE D MAGE D2 antigen MAGE-D2 antigen MAGED2 Melanoma antigen family D 2 Melanoma associated antigen D2 Melanoma-associated antigen D2
Images
HA751566_1.jpg Fig1: Western blot analysis of MAGED2 on different lysates with Rabbit anti-MAGED2 antibody (HA751566) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: A431 cell lysate
Lane 3: A549 cell lysate
Lane 4: U-2 OS cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 65 kDa
Observed band size: 72 kDa

Exposure time: 1 minute 18 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751566) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.