Ryanodine receptor 2 / RYR-2 Recombinant Rabbit Monoclonal Antibody [PSH15-54]
cat.: HA751567
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH15-54 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 565 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human RYR-2 aa 1,301-1,600. |
| Positive control: | Mouse heart tissue lysate, Rat heart tissue lysate, mouse heart tissue. |
| Subcellular location: | Sarcoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200 |
| Uniprot #: | SwissProt: E9Q401 Mouse | B0LPN4 Rat |
| Alternative names: | ARVC2 ARVD2 Cardiac muscle ryanodine receptor Cardiac muscle ryanodine receptor-calcium release channel hRYR-2 ryanodine receptor 2 (cardiac) Ryanodine receptor 2 RyR RYR-2 RyR2 RYR2_HUMAN Type 2 ryanodine receptor VTSIP |
Images
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Fig1:
Western blot analysis of Ryanodine receptor 2 / RYR-2 on different lysates with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (HA751567) at 1/2,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Mouse skeletal muscle tissue lysate (negative) Lane 3: Mouse liver tissue lysate (negative) Lane 4: Rat heart tissue lysate Lane 5: Rat skeletal muscle tissue lysate (negative) Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 40 µg/Lane. Predicted band size: 565 kDa Observed band size: 565 kDa Exposure time: 1 minute 7 seconds; ECL: K1802; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751567) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (HA751567) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751567) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (HA751567) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751567) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.