TAZ / WWTR1 Recombinant Rabbit Monoclonal Antibody [PSH15-58]
cat.: HA751569
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH15-58 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TAZ / WWTR1 aa 1-400. |
| Positive control: | HeLa cell lysate, PANC-1 cell lysate, SK-MEL-28 cell lysate, A375 cell lysate, F9 cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, human breast carcinoma tissue, human kidney tissue, human stomach tissue, mouse kidney tissue, mouse stomach tissue, rat kidney tissue, rat stomach tissue. |
| Subcellular location: | Nucleus, Cytoplasm, Cell membrane, Cell junction, tight junction. |
| Recommended Dilutions:
WB IHC-P IP |
1:5,000 1:200-1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q9GZV5 Human | Q9EPK5 Mouse Entrez Gene: 295062 Rat |
| Alternative names: | DKFZP586I1419 FLJ27004 FLJ45718 OTTHUMP00000215994 OTTHUMP00000215995 OTTHUMP00000215996 OTTHUMP00000216001 TAZ Transcriptional co activator with PDZ binding motif Transcriptional coactivator with PDZ binding motif Transcriptional coactivator with PDZ-binding motif WW domain containing transcription regulator 1 WW domain containing transcription regulator protein 1 WW domain-containing transcription regulator protein 1 WWTR 1 WWTR1 WWTR1_HUMAN |
Images
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Fig1:
Western blot analysis of TAZ / WWTR1 on different lysates with Rabbit anti-TAZ / WWTR1 antibody (HA751569) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PANC-1 cell lysate Lane 3: SH-SY5Y cell lysate (low expression) Lane 4: SK-MEL-28 cell lysate Lane 5: Raji cell lysate (negative) Lane 6: A375 cell lysate Lane 7: F9 cell lysate Lane 8: C2C12 cell lysate Lane 9: NIH/3T3 cell lysate Lane 10: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 50 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751569) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-TAZ / WWTR1 antibody (HA751569) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751569) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-TAZ / WWTR1 antibody (HA751569) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751569) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-TAZ / WWTR1 antibody (HA751569) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751569) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TAZ / WWTR1 antibody (HA751569) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751569) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-TAZ / WWTR1 antibody (HA751569) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751569) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-TAZ / WWTR1 antibody (HA751569) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751569) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-TAZ / WWTR1 antibody (HA751569) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751569) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9:
TAZ / WWTR1 was immunoprecipitated from 0.2 mg PANC-1 cell lysate with HA751569 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751569 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: PANC-1 cell lysate (input) Lane 2: HA751569 IP in PANC-1 cell lysate Lane 3: Rabbit IgG instead of HA751569 in PANC-1 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 minutes; ECL: K1801 |
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Fig10:
TAZ / WWTR1 was immunoprecipitated from 0.2 mg C2C12 cell lysate with HA751569 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751569 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: C2C12 cell lysate (input) Lane 2: HA751569 IP in C2C12 cell lysate Lane 3: Rabbit IgG instead of HA751569 in C2C12 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 20 seconds; ECL: K1801 |
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