Endothelin 1 Recombinant Rabbit Monoclonal Antibody [PSH15-63]
cat.: HA751574
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH15-63 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Endothelin 1 aa 1-212. |
| Positive control: | HUVEC cell lysate, MDA-MB-231 cell lysate, PC-3M cell lysate, Saos-2 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P05305 Human | P22387 Mouse | P22388 Rat |
| Alternative names: | ARCND3 Big endothelin-1 EDN1 EDN1_HUMAN ET-1 ET1 HDLCQ7 PPET1 Preproendothelin 1 Preproendothelin-1 QME |
Images
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Fig1:
Western blot analysis of Endothelin 1 on different lysates with Rabbit anti-Endothelin 1 antibody (HA751574) at 1/5,000 dilution. Lane 1: HUVEC cell lysate Lane 2: MDA-MB-231 cell lysate Lane 3: PC-3M cell lysate Lane 4: Saos-2 cell lysate Lane 5: HeLa cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 24 kDa Observed band size: 21 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751574) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.