IL-1 alpha Recombinant Rabbit Monoclonal Antibody [PSH15-65]
cat.: HA751576
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH15-65 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse IL-1 alpha aa 115-270. |
| Positive control: | RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate. |
| Subcellular location: | Nucleus, Cytoplasm, Secreted. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P01582 Mouse |
| Alternative names: | BAF FAF Hematopoietin 1 Hematopoietin-1 IL 1 alpha IL 1A IL-1 alpha Il-1a IL1 ALPHA IL1 IL1A IL1A_HUMAN IL1F1 Interleukin 1 alpha Interleukin-1 alpha Interleukin1 alpha LAF LEM Preinterleukin 1 alpha Pro interleukin 1 alpha |
Images
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Fig1:
Western blot analysis of IL-1 alpha on different lysates with Rabbit anti-IL-1 alpha antibody (HA751576) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751576) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.