SIRT5 Recombinant Rabbit Monoclonal Antibody [PSH15-71]
cat.: HA751578
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH15-71 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Positive control: | 293T cell lysate, Hepa-1-6 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Rat liver tissue lysate, human liver tissue. |
| Subcellular location: | Mitochondrion matrix, Mitochondrion intermembrane space, Cytoplasm, cytosol, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:1,000 |
| Uniprot #: | SwissProt: Q9NXA8 Human | Q8K2C6 Mouse | Q68FX9 Rat |
| Alternative names: | NAD dependent deacetylase sirtuin 5 NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial NAD dependent protein deacylase sirtuin 5 mitochondrial NAD-dependent protein deacylase sirtuin-5, mitochondrial Regulatory protein SIR2 homolog 5 Silent mating type information regulation 2 S.cerevisiae homolog 5 Sir2 like 5 SIR2-like protein 5 SIR2L5 SIR5_HUMAN Sirt5 Sirtuin 5 Sirtuin type 5 |
Images
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Fig1:
Western blot analysis of SIRT5 on different lysates with Rabbit anti-SIRT5 antibody (HA751578) at 1/2,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: Hepa-1-6 cell lysate (20 µg/Lane) Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: Rat liver tissue lysate (20 µg/Lane) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751578) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-SIRT5 antibody (HA751578) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751578) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.