UCP1 Recombinant Rabbit Monoclonal Antibody [PSH15-73]
cat.: HA751580
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH15-73 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Positive control: | Mouse brown adipose tissue lysate, Rat brown adipose tissue lysate, mouse brown adipose tissue, rat brown adipose tissue. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IHC-Fr IHC-P IP |
1:2,000 1:500 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P12242 Mouse | P04633 Rat |
| Alternative names: | mitochondrial brown fat uncoupling protein Mitochondrial brown fat uncoupling protein 1 SLC25A7 Solute carrier family 25 member 7 Thermogenin UCP 1 UCP UCP1 UCP1_HUMAN uncoupling protein 1 (mitochondrial, proton carrier) Uncoupling protein 1 |
Images
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Fig1:
Western blot analysis of UCP1 on different lysates with Rabbit anti-UCP1 antibody (HA751580) at 1/2,000 dilution. Lane 1: Mouse brown adipose tissue lysate Lane 2: Rat brown adipose tissue lysate Lane 3: Rat white adipose tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751580) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Application: IHC-Fr Species: Mouse Site: brown adipose Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue with Rabbit anti-UCP1 antibody (HA751580) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751580) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse white adipose tissue (negative) with Rabbit anti-UCP1 antibody (HA751580) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751580) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue with Rabbit anti-UCP1 antibody (HA751580) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751580) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded rat white adipose tissue (negative) with Rabbit anti-UCP1 antibody (HA751580) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751580) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
UCP1 was immunoprecipitated from 0.2 mg mouse brown adipose tissue lysate with HA751580 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751580 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: mouse brown adipose tissue lysate (input) Lane 2: HA751580 IP in mouse brown adipose tissue lysate Lane 3: Rabbit IgG instead of HA751580 in mouse brown adipose tissue lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.