Granzyme K Recombinant Rabbit Monoclonal Antibody [PSH16-28]
cat.: HA751596
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH16-28 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Granzyme K aa 1-264. |
| Positive control: | NK-92 cell lysate, human NK/T lymphoma tissue. |
| Subcellular location: | Secreted, Cytoplasmic granule. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:5,000 1:1,000 1:500 |
| Uniprot #: | SwissProt: P49863 Human |
| Alternative names: | Fragmentin 3 Fragmentin-3 Fragmentin3 GRAK_HUMAN Granzyme 3 granzyme K (granzyme 3; tryptase II) granzyme K (serine protease, granzyme 3; tryptase II) Granzyme K Granzyme K precursor Granzyme-3 Granzyme3 GranzymeK GZMK NK TRYP 2 NK TRYP2 NK tryptase 2 NK-TRYP-2 NK-tryptase-2 NKTRYP2 Serine protease granzyme 3 TRYP 2 TRYP2 Tryptase II TryptaseII |
Images
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Fig1:
Western blot analysis of Granzyme K on different lysates with Rabbit anti-Granzyme K antibody (HA751596) at 1/5,000 dilution. Lane 1: NK-92 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (negative) (20 µg/Lane) Predicted band size: 29 kDa Observed band size: 27 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751596) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human NK/T lymphoma tissue with Rabbit anti-Granzyme K antibody (HA751596) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751596) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue (negative) with Rabbit anti-Granzyme K antibody (HA751596) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751596) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Application: IF-Tissue Species: Human Site: NK/T lymphoma Sample: Paraffin-embedded section Antibody concentration: 1/500 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.