TIM 3 Recombinant Rabbit Monoclonal Antibody [PSH16-50]
cat.: HA751599
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH16-50 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Positive control: | RPMI 8226 cell lysate, RAW264.7 cell lysates, human lung cancer tissue, mouse spleen tissue, rat spleen tissue. |
| Subcellular location: | Membrane, Cell junction, Cell membrane. |
| Recommended Dilutions:
WB IHC-P IP |
1:2,000 1:200-1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q8TDQ0 Human | Q8VIM0 Mouse | P0C0K5 Rat |
| Alternative names: | CD366 FLJ14428 HAVcr-2 Havcr2 HAVR2_HUMAN Hepatitis A virus cellular receptor 2 Kidney injury molecule 3 KIM 3 KIM3 T cell immunoglobulin and mucin domain containing 3 T cell immunoglobulin mucin 3 T-cell immunoglobulin and mucin domain-containing protein 3 T-cell immunoglobulin mucin family member 3 T-cell immunoglobulin mucin receptor 3 T-cell membrane protein 3 Tim 3 TIM-3 TIM3 TIMD-3 TIMD3 |
Images
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Fig1:
Western blot analysis of TIM 3 on different lysates with Rabbit anti-TIM 3 antibody (HA751599) at 1/2,000 dilution. Lane 1: RPMI 8226 cell lysate (20 µg/Lane) Lane 2: HT-29 cell lysate (negative) (20 µg/Lane) Predicted band size: 33 kDa Observed band size: 55 kDa Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751599) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of TIM 3 on RAW264.7 cell lysates with Rabbit anti-TIM 3 antibody (HA751599) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 70 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751599) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-TIM 3 antibody (HA751599) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751599) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TIM 3 antibody (HA751599) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751599) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TIM 3 antibody (HA751599) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751599) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
TIM 3 was immunoprecipitated from 0.2 mg RPMI 8226 cell lysate with HA751599 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751599 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: RPMI 8226 cell lysate (input) Lane 2: HA751599 IP in RPMI 8226 cell lysate Lane 3: Rabbit IgG instead of HA751599 in RPMI 8226 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 seconds; ECL: K1801 |
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Fig7:
TIM 3 was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with HA751599 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751599 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: RAW264.7 cell lysate (input) Lane 2: HA751599 IP in RAW264.7 cell lysate Lane 3: Rabbit IgG instead of HA751599 in RAW264.7 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 46 seconds; ECL: K1802 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.