TRPV4 Recombinant Rabbit Monoclonal Antibody [PSH16-51]
cat.: HA751600
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-Fr, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH16-51 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 98 kDa |
| Isotype: | IgG |
| Positive control: | A375 cell lysate, mouse kidney tissue. |
| Subcellular location: | Cell membrane, Apical cell membrane, Cell junction, adherens junction, Cell projection, cilium. |
| Recommended Dilutions:
WB IHC-Fr IHC-P IP |
1:1,000 1:500 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q9HBA0 Human | Q9EPK8 Mouse |
| Alternative names: | BCYM3 CMT2C HMSN2C osm 9 like TRP channel 4 Osm-9-like TRP channel 4 OSM9 like transient receptor potential channel 4 Osmosensitive transient receptor potential channel 4 OTRPC 4 OTRPC4 SMAL SPSMA SSQTL1 Transient receptor potential cation channel subfamily V member 4 Transient receptor potential protein 12 TRP 12 TRP12 TRPV 4 TrpV4 TRPV4_HUMAN Vanilloid receptor like channel 2 Vanilloid receptor like protein 2 Vanilloid receptor related osmotically activated channel Vanilloid receptor-like channel 2 Vanilloid receptor-like protein 2 Vanilloid receptor-related osmotically-activated channel VR 4 VR OAC VR-OAC VR4 VRL 2 VRL-2 VRL2 VROAC |
Images
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Fig1:
Western blot analysis of TRPV4 on different lysates with Rabbit anti-TRPV4 antibody (HA751600) at 1/1,000 dilution. Lane 1: A375 cell lysate Lane 2: 293T cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 98 kDa Observed band size: 90 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751600) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Application: IHC-Fr Species: Mouse Site: kidney Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TRPV4 antibody (HA751600) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751600) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
TRPV4 was immunoprecipitated from 0.2 mg A375 cell lysate with HA751600 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751600 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A375 cell lysate (input) Lane 2: HA751600 IP in A375 cell lysate Lane 3: Rabbit IgG instead of HA751600 in A375 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 59 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.