Neuropeptide Y Recombinant Rabbit Monoclonal Antibody [PSH16-52]
cat.: HA751601
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-Fr, IHC-P, WB |
| Clonality: | Monoclonal |
| Clone number: | PSH16-52 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 11 kDa |
| Isotype: | IgG |
| Positive control: | Human cerebrum tissue, mouse cerebrum tissue, rat cerebrum tissue, SH-SY5Y treated with 200nM TPA for 24 hours cell lysate, HEK-293 transfected with Neuropeptide Y then treated with 200nM TPA for 24 hours cell lysate. |
| Subcellular location: | Secreted, Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle. |
| Recommended Dilutions:
IHC-Fr IHC-P WB |
1:500 1:1,000 1:2,000 |
| Uniprot #: | SwissProt: P01303 Human | P57774 Mouse | P07808 Rat |
| Alternative names: | C-flanking peptide of NPY CPON Neuropeptide tyrosine Neuropeptide Y precursor NPY NPY_HUMAN Pro neuropeptide Y PYY 4 PYY4 Y Neuropeptide |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Rat Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue with Rabbit anti-Neuropeptide Y antibody (HA751601) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751601) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-Neuropeptide Y antibody (HA751601) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751601) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue with Rabbit anti-Neuropeptide Y antibody (HA751601) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751601) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Western blot analysis of Neuropeptide Y on different lysates with Rabbit anti-Neuropeptide Y antibody (HA751601) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM TPA for 24 hours cell lysate Lane 3: HEK-293 transfected with Neuropeptide Y cell lysate Lane 4: HEK-293 transfected with Neuropeptide Y, then treated with 200nM TPA for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 11 kDa Observed band size: 15 kDa Exposure time: Lane 1-2: 59 seconds; Lane 3-4: 10 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751601) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.