Orexin Recombinant Rabbit Monoclonal Antibody [PSH16-77]
cat.: HA751606
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH16-77 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Positive control: | Mouse hypothalamus tissue, rat hypothalamus tissue. |
| Subcellular location: | Rough endoplasmic reticulum, Cytoplasmic vesicle, Synapse. |
| Recommended Dilutions:
IHC-Fr IHC-P |
1:100 1:100 |
| Uniprot #: | SwissProt: O55241 Mouse | O55232 Rat |
| Alternative names: | HCRT HCRT1 HCRT2 hypocretin (orexin) neuropeptide precursor Hypocretin 1 Hypocretin 2 hypocretin NRCLP1 Orexin Orexin A Orexin B Orexin precursor OX PPORX PPOX PREPROOREXIN |
Images
|
Fig1:
Application: IHC-Fr Species: Mouse Site: hypothalamus Sample: Frozen section Antibody concentration: 1/100 Antigen retrieval: Not required |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue with Rabbit anti-Orexin antibody (HA751606) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751606) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue with Rabbit anti-Orexin antibody (HA751606) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751606) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.