HGF Recombinant Rabbit Monoclonal Antibody [PSH03-79]
cat.: HA751614
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH03-79 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 83 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human HGF aa 1-728. |
| Positive control: | U-87 MG cell lysate, NCI-H1299 cell lysate. |
| Subcellular location: | Extracellular region, Extracellular space. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P14210 Human |
| Alternative names: | DFNB39 F TCF Fibroblast derived tumor cytotoxic factor Hepatocyte growth factor (hepapoietin A; scatter factor) Hepatocyte growth factor Hepatocyte growth factor beta chain Hepatocyte growth factor precursor Hepatopoietin A Hepatopoietin-A Hgf HGF_HUMAN HGFB HPTA Lung fibroblast derived mitogen OTTHUMP00000161349 OTTHUMP00000206710 OTTHUMP00000206711 OTTHUMP00000206712 OTTHUMP00000206713 OTTHUMP00000206730 Scatter factor SF |
Images
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Fig1:
Western blot analysis of HGF on different lysates with Rabbit anti-HGF antibody (HA751614) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: NCI-H1299 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751614) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.