GABRA2 Recombinant Rabbit Monoclonal Antibody [PSH17-28]
cat.: HA751620
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P, WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH17-28 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Positive control: | Mouse hippocampus tissue, rat hippocampus tissue, Mouse brain tissue lysate, Rat cerebellum tissue lysate. |
| Subcellular location: | Postsynaptic cell membrane, Cell membrane, Cytoplasmic vesicle membrane, Cell projection, dendrite. |
| Recommended Dilutions:
IHC-Fr IHC-P WB IP |
1:500 1:200-1:1,000 1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: P26048 Mouse | P23576 Rat |
| Alternative names: | GABA A receptor subunit alpha 2 GABA GABA(A) receptor subunit alpha 2 GABA(A) receptor subunit alpha-2 GABR A2 GABRA 2 GABRA2 GABRA2 protein Gamma aminobutyric acid (GABA) A receptor alpha 2 Gamma aminobutyric acid A receptor alpha 2 Gamma aminobutyric acid receptor alpha 2 subunit Gamma aminobutyric acid receptor subunit alpha 2 Gamma-aminobutyric acid receptor subunit alpha-2 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Rat Site: hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-GABRA2 antibody (HA751620) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751620) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-GABRA2 antibody (HA751620) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751620) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Western blot analysis of GABRA2 on different lysates with Rabbit anti-GABRA2 antibody (HA751620) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat cerebellum tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751620) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig6:
GABRA2 was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA751620 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751620 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: mouse brain tissue lysate (input) Lane 2: HA751620 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of HA751620 in mouse brain tissue lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 10 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.