ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH17-38]
cat.: HA751626
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | IHC-Fr, IHC-P, WB, IF-Cell, FC, IP, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH17-38 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse ICAM1 aa 1-485. |
| Positive control: | Mouse lung tissue, RAW264.7 cell lysate, Mouse spleen tissue lysate, Mouse kidney tissue lysate, RAW264.7, mouse spleen cells. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
IHC-Fr IHC-P WB IF-Cell FC IP IF-Tissue |
1:1,000 1:2,000 1:10,000 1:200 1:2,000 1-2μg/sample 1:500 |
| Uniprot #: | SwissProt: P13597 Mouse |
| Alternative names: | Antigen identified by monoclonal BB2 BB 2 BB2 CD 54 CD_antigen=CD54 CD54 Cell surface glycoprotein P3.58 Human rhinovirus receptor ICAM 1 ICAM-1 ICAM1 ICAM1_HUMAN intercellular adhesion molecule 1 (CD54), human rhinovirus receptor Intercellular adhesion molecule 1 Major group rhinovirus receptor MALA 2 MALA2 MyD 10 MyD10 P3.58 Surface antigen of activated B cells, BB2 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: small intestine Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-ICAM1 antibody (HA751626) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751626) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (HA751626) at 1/10,000 dilution. Lane 1: RAW264.7 cell lysate (20 µg/Lane) Lane 2: Mouse spleen tissue lysate (30 µg/Lane) Lane 3: Mouse kidney tissue lysate (30 µg/Lane) Predicted band size: 60 kDa Observed band size: 120 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751626) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunocytochemistry analysis of RAW264.7 cells labeling ICAM1 with Rabbit anti-ICAM1 antibody (HA751626) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ICAM1 antibody (HA751626) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Flow cytometric analysis of mouse spleen cells labeling ICAM1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751626, 1/2,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6:
ICAM1 was immunoprecipitated from 0.2 mg mouse spleen tissue lysate with HA751626 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751626 at 1/10,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse spleen tissue lysate (input) Lane 2: HA751626 IP in mouse spleen tissue lysate Lane 3: Rabbit IgG instead of HA751626 in mouse spleen tissue lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 59 seconds; ECL: K1801 |
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Fig7:
Application: Immunofluorescence (IF-tissue) Species: Mouse Tissue: Lung Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA751626, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.