CBL Recombinant Rabbit Monoclonal Antibody [PSH17-61]
cat.: HA751635
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IF-Cell, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH17-61 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 100 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CBL aa 441-840. |
| Positive control: | Raji cell lysate, K-562 cell lysate, COS-1 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, Raji. |
| Subcellular location: | Cytoplasm, Cell membrane, Cell projection, cilium, Golgi apparatus. |
| Recommended Dilutions:
WB IF-Cell FC IP |
1:5,000 1:100 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P22681 Human | P22682 Mouse Entrez Gene: 500985 Rat |
| Alternative names: | 4732447J05Rik C CBL Cas Br M (murine) ecotropic retroviral transforming sequence Casitas B lineage lymphoma proto oncogene Casitas B-lineage lymphoma proto-oncogene CBL 2 cbl CBL_HUMAN CBL2 E3 ubiquitin protein ligase CBL E3 ubiquitin-protein ligase CBL Oncogene CBL2 Proto oncogene c CBL Proto-oncogene c-CBL RGD1561386 RING finger protein 55 RNF55 Signal transduction protein CBL |
Images
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Fig1:
Western blot analysis of CBL on different lysates with Rabbit anti-CBL antibody (HA751635) at 1/5,000 dilution. Lane 1: Raji cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: COS-1 cell lysate (20 µg/Lane) Lane 4: Mouse testis tissue lysate (30 µg/Lane) Lane 5: Rat testis tissue lysate (30 µg/Lane) Predicted band size: 100 kDa Observed band size: 110 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751635) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Raji cells labeling CBL with Rabbit anti-CBL antibody (HA751635) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CBL antibody (HA751635) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of Raji cells labeling CBL. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751635, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
CBL was immunoprecipitated from 0.2 mg Raji cell lysate with HA751635 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751635 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Raji cell lysate (input) Lane 2: HA751635 IP in Raji cell lysate Lane 3: Rabbit IgG instead of HA751635 in Raji cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 10 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.