intestinal alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [PSH17-82]
cat.: HA751641
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH17-82 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ALPI aa 1-503. |
| Positive control: | Saos-2 cell lysate, Mouse small intestine tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P09923 Human |
| Alternative names: | Alkaline phosphatase intestinal Alkaline phosphomonoesterase Alpi Glycerophosphatase IAP Intestinal alkaline phosphatase Intestinal-type alkaline phosphatase Kasahara isozyme OTTHUMP00000164357 PPBI_HUMAN |
Images
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Fig1:
Western blot analysis of intestinal alkaline phosphatase on different lysates with Rabbit anti-intestinal alkaline phosphatase antibody (HA751641) at 1/5,000 dilution. Lane 1: Saos-2 cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (negative) (20 µg/Lane) Lane 3: Mouse small intestine tissue lysate (30 µg/Lane) Predicted band size: 57 kDa Observed band size: 80 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751641) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.