BCMA Recombinant Rabbit Multiclonal Antibody [PSH18-02]
cat.: HA751650
| Product Type: | Recombinant Rabbit multiclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell, FC |
| Clone number: | PSH18-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Positive control: | U266. |
| Subcellular location: | Cell membrane, Endomembrane system. |
| Recommended Dilutions:
IF-Cell FC |
1:100 1μg/mL |
| Uniprot #: | SwissProt: Q02223 Human |
| Alternative names: | B cell maturation antigen B cell maturation factor B cell maturation protein B-cell maturation protein BCM BCMA CD269 CD269 antigen TNFRSF17 TNR17_HUMAN Tumor necrosis factor receptor superfamily member 17 |
Images
|
Fig1:
Immunocytochemistry analysis of U266 (positive) and Jurkat (negative) labeling BCMA with Rabbit anti-BCMA antibody (HA751650) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BCMA antibody (HA751650) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig2:
Flow cytometric analysis of Jurkat (left, negative) and U266 (right, positive) cells labeling BCMA. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751650, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.