PADI2 Recombinant Rabbit Monoclonal Antibody [PSH18-07]
cat.: HA751653
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Monkey |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH18-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 76 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PADI2 aa 1-350. |
| Positive control: | PANC-1 cell lysate, SK-Br-3 cell lysate, MCF7 cell lysate, HT-29 cell lysate, COS-1 cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IP |
1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q9Y2J8 Human |
| Alternative names: | KIAA0994 OTTHUMP00000044625 PAD 2 PAD H19 PAD-H19 PAD2 PADI 2 Padi2 PADI2 protein PADI2_HUMAN PDI 2 PDI2 Peptidlyarginine deiminase type II Peptidyl arginine deiminase II Peptidyl arginine deiminase type II Peptidylarginine deiminase II Protein arginine deiminase Protein arginine deiminase type 2 Protein arginine deiminase type II Protein-arginine deiminase type II Protein-arginine deiminase type-2 |
Images
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Fig1:
Western blot analysis of PADI2 on different lysates with Rabbit anti-PADI2 antibody (HA751653) at 1/5,000 dilution. Lane 1: PANC-1 cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: MCF7 cell lysate (low expression) Lane 4: HT-29 cell lysate Lane 5: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 76 kDa Observed band size: 75 kDa Exposure time: Lane 1-2: 6 seconds; Lane 3-5: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751653) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
PADI2 was immunoprecipitated from 0.2 mg PANC-1 cell lysate with HA751653 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751653 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: PANC-1 cell lysate (input) Lane 2: HA751653 IP in PANC-1 cell lysate Lane 3: Rabbit IgG instead of HA751653 in PANC-1 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 2 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.