IL-18 Recombinant Rabbit Monoclonal Antibody [PSH18-15]
cat.: HA751661
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH18-15 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse IL-18 aa 1-192. |
| Positive control: | HeLa cell lysate, RAW264.7 cell lysate, J774A.1 cell lysate, C6 cell lysate, Mouse liver tissue lysate, Mouse thymus tissue lysate, Rat liver tissue lysate, mouse liver tissue, mouse thymus tissue, rat thymus tissue. |
| Subcellular location: | Cytoplasm, cytosol, Secreted. |
| Recommended Dilutions:
WB IHC-P IP |
1:5,000 1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q14116 Human | P70380 Mouse | P97636 Rat |
| Alternative names: | Iboctadekin IFN gamma inducing factor IFN-gamma-inducing factor IGIF IL 1 gamma IL 18 IL 1g IL-1 gamma IL-18 IL1 gamma IL18 IL18 protein IL18_HUMAN IL1F4 IL1g IL1gamma ILIF4 Interferon gamma inducing factor Interferon gamma-inducing factor Interleukin 1 gamma Interleukin 18 (interferon-gamma-inducing factor) Interleukin 18 Interleukin-1 gamma Interleukin-18 Interleukin18 MGC12320 |
Images
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Fig1:
Western blot analysis of IL-18 on different lysates with Rabbit anti-IL-18 antibody (HA751661) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate (negative) Lane 3: RAW264.7 cell lysate Lane 4: J774A.1 cell lysate Lane 5: C6 cell lysate Lane 6: Mouse liver tissue lysate Lane 7: Mouse thymus tissue lysate Lane 8: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751661) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-IL-18 antibody (HA751661) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751661) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-IL-18 antibody (HA751661) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751661) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat thymus tissue with Rabbit anti-IL-18 antibody (HA751661) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751661) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
IL-18 was immunoprecipitated from 0.2 mg J774A.1 cell lysate with HA751661 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751661 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: J774A.1 cell lysate (input) Lane 2: HA751661 IP in J774A.1 cell lysate Lane 3: Rabbit IgG instead of HA751661 in J774A.1 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 10 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.