Myeloperoxidase Recombinant Rabbit Monoclonal Antibody [PSH18-17]
cat.: HA751663
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH18-17 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Myeloperoxidase aa 1-718. |
| Positive control: | HL-60 cell lysate, Mouse spleen tissue lysate, Rat bone marrow tissue lysate, human bone marrow tissue, human spleen tissue, mouse spleen tissue, rat bone marrow tissue, rat spleen tissue. |
| Subcellular location: | Lysosome. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P05164 Human | P11247 Mouse Entrez Gene: 303413 Rat |
| Alternative names: | 84 kDa myeloperoxidase 89 kDa myeloperoxidase EC 1.11.1.7 EC1.11.2.2 fj80f04 MPO mpx myeloid-specific peroxidase Myeloperoxidase Myeloperoxidase heavy chain Myeloperoxidase light chain PERM_HUMAN wu:fj80f04 |
Images
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Fig1:
Western blot analysis of Myeloperoxidase on different lysates with Rabbit anti-Myeloperoxidase antibody (HA751663) at 1/5,000 dilution. Lane 1: HL-60 cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (negative) (20 µg/Lane) Lane 3: Mouse spleen tissue lysate (40 µg/Lane) Lane 4: Rat bone marrow tissue lysate (40 µg/Lane) Predicted band size: 84 kDa Observed band size: 84/55/37 kDa Exposure time: Lane 1-2: 3 minutes; Lane 3-4: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751663) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Rabbit anti-Myeloperoxidase antibody (HA751663) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751663) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Myeloperoxidase antibody (HA751663) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751663) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Myeloperoxidase antibody (HA751663) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751663) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat bone marrow tissue with Rabbit anti-Myeloperoxidase antibody (HA751663) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751663) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Myeloperoxidase antibody (HA751663) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751663) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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