IGFBP1 Recombinant Rabbit Monoclonal Antibody [PSH18-18]
cat.: HA751664
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH18-18 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human 1-259. |
| Positive control: | HepG2 cell lysate, MDA-MB-231 cell lysate, HepG2. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell FC IP |
1:5,000 1:100 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P08833 Human |
| Alternative names: | AFBP Alpha pregnancy associated endometrial globulin Amniotic fluid binding protein Binding protein 25 Binding protein 26 Binding protein 28 Growth hormone independent binding protein hIGFBP 1 hIGFBP1 IBP 1 IBP-1 IBP1 IBP1_HUMAN IGF binding protein 1 IGF BP25 IGF-binding protein 1 IGFBP 1 IGFBP-1 IGFBP1 Insulin like growth factor binding protein 1 Insulin-like growth factor-binding protein 1 Placental protein 12 PP 12 PP12 |
Images
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Fig1:
Western blot analysis of IGFBP1 on different lysates with Rabbit anti-IGFBP1 antibody (HA751664) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: SK-MEL-28 cell lysate (negative) (30 µg/Lane) Lane 3: MDA-MB-231 cell lysate (30 µg/Lane) Predicted band size: 28 kDa Observed band size: 34 kDa Exposure time: Lane 1: 20 seconds; Lane 2-3: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751664) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HepG2 (positive) and SK-MEL-28 (negative) labeling IGFBP1 with Rabbit anti-IGFBP1 antibody (HA751664) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IGFBP1 antibody (HA751664) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of SK-MEL-28 (left, negative) and HepG2 (right, positive) cells labeling IGFBP1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751664, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
IGFBP1 was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA751664 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751664 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input) Lane 2: HA751664 IP in HepG2 cell lysate Lane 3: Rabbit IgG instead of HA751664 in HepG2 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 20 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.