MAP4K4 / NIK Recombinant Rabbit Monoclonal Antibody [PSH18-45]
cat.: HA751669
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH18-45 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 142 kDa |
| Isotype: | IgG |
| Positive control: | Human kidney tissue, human liver tissue, human skeletal muscle tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
IHC-P |
1:200-1:1,000 |
| Uniprot #: | SwissProt: O95819 Human |
| Alternative names: | FLH21957 FLJ10410 FLJ20373 FLJ90111 Hepatocyte progenitor kinase like/germinal center kinase like kinase HGK HPK/GCK like kinase HPK/GCK like kinase HGK HPK/GCK-like kinase HGK KIAA0687 M4K4_HUMAN MAP4K4 MAPK/ERK kinase kinase kinase 4 MEK kinase kinase 4 MEKKK 4 MEKKK4 Mitogen activated protein kinase kinase kinase kinase 4 Mitogen-activated protein kinase kinase kinase kinase 4 Nck interacting kinase Nck-interacting kinase NIK |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-MAP4K4 / NIK antibody (HA751669) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751669) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-MAP4K4 / NIK antibody (HA751669) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751669) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-MAP4K4 / NIK antibody (HA751669) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751669) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.