Ly6g Recombinant Rabbit Monoclonal Antibody [PSH18-48]
cat.: HA751672
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH18-48 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Ly6g aa 1-105. |
| Positive control: | Mouse spleen tissue lysate, Mouse lung tissue lysate, mouse bone marrow tissue, mouse spleen tissue, mouse lung tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:5,000-1:10,000 |
| Uniprot #: | SwissProt: P35461 Mouse |
| Alternative names: | Gr-1 Gr1 Ly-6G.1 Ly6G Lymphocyte antigen 6 complex locus G Lymphocyte antigen 6G |
Images
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Fig1:
Western blot analysis of Ly6g on different lysates with Rabbit anti-Ly6g antibody (HA751672) at 1/5,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse lung tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 14 kDa Observed band size: 25 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751672) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse bone marrow tissue with Rabbit anti-Ly6g antibody (HA751672) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751672) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Ly6g antibody (HA751672) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751672) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Ly6g antibody (HA751672) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751672) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Western blot analysis of Ly6g on different lysates with Rabbit anti-Ly6g antibody (HA751672) at 1/5,000 dilution. Lane 1: 293T transfected with empty control cell lysate (negative) Lane 2: 293T transfected with Ly6g cell lysate (positive) Lane 3: 293T transfected with Ly6f cell lysate (negative) Lane 4: 293T transfected with Ly6i cell lysate (negative) Lane 5: 293T transfected with Ly6c1 cell lysate (negative) Lane 6: 293T transfected with Ly6c2 cell lysate (negative) Lysates/proteins at 10 µg/Lane. Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751672) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.