AKR1B10 Recombinant Rabbit Monoclonal Antibody [PSH18-56]
cat.: HA751680
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH18-56 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human AKR1B10 aa 101-150. |
| Positive control: | HepG2 cell lysate, HT-29 cell lysate, A549 cell lysate, T98G cell lysate, A549. |
| Subcellular location: | Lysosome, Secreted. |
| Recommended Dilutions:
WB IP IF-Cell FC |
1:10,000-1:200,000 1-2μg/sample 1:250 1:1,000 |
| Uniprot #: | SwissProt: O60218 Human |
| Alternative names: | Aldo-keto reductase family 1 member B10 EC:1.1.1.300 EC:1.1.1.54 ARL-1 Aldose reductase-like Aldose reductase-related protein ARP hARP Small intestine reductase SI reductase AKR1B10 AKR1B11 |
Images
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Fig1:
Western blot analysis of AKR1B10 on different lysates with Rabbit anti-AKR1B10 antibody (HA751680) at 1/10,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HT-29 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 35 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751680) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of AKR1B10 on different lysates with Rabbit anti-AKR1B10 antibody (HA751680) at 1/200,000 dilution. Lane 1: A549 cell lysate Lane 2: T98G cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 35 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751680) at 1/200,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
AKR1B10 was immunoprecipitated from 0.2 mg A549 cell lysate with HA751680 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751680 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A549 cell lysate (input) Lane 2: HA751680 IP in A549 cell lysate Lane 3: Rabbit IgG instead of HA751680 in A549 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 2 seconds; ECL: K1801 |
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Fig4:
Immunocytochemistry analysis of A549 cells labeling AKR1B10 with Rabbit anti-AKR1B10 antibody (HA751680) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKR1B10 antibody (HA751680) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Flow cytometric analysis of A549 cells labeling AKR1B10. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751680, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.