FGF1 Recombinant Rabbit Monoclonal Antibody [PSH18-61]
cat.: HA751685
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, FC
Clonality: Monoclonal
Clone number: PSH18-61
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 18 kDa
Isotype: IgG
Immunogen: Recombinant protein within human FGF1 aa 1-155.
Positive control: U-87 MG cell lysate, U-2 OS cell lysate, Neuro-2a cell lysate, Mouse kidney tissue lysate, Mouse heart tissue lysate, Rat heart tissue lysate, U-87 MG, Neuro-2a.
Subcellular location: Secreted, Cytoplasm, cell cortex, cytosol, Nucleus.
Recommended Dilutions:
  WB
  FC
1:2,000-1:5,000
1:1,000
Uniprot #: SwissProt: P05230 Human | P61148 Mouse | P61149 Rat
Alternative names: Acidic fibroblast growth factor aFGF Beta endothelial cell growth factor Beta-endothelial cell growth factor ECGF ECGF beta ECGF-beta ECGFA ECGFB Endothelial Cell Growth Factor alpha Endothelial Cell Growth Factor beta FGF 1 FGF alpha Fgf1 FGF1_HUMAN FGFA Fibroblast Growth Factor 1 Acidic Fibroblast growth factor 1 GLIO703 HBGF 1 HBGF-1 HBGF1 Heparin binding growth factor 1 Heparin binding growth factor 1 precursor Heparin-binding growth factor 1
Images
HA751685_1.jpg Fig1: Western blot analysis of FGF1 on different lysates with Rabbit anti-FGF1 antibody (HA751685) at 1/2,000 dilution.

Lane 1: U-87 MG cell lysate
Lane 2: U-2 OS cell lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 17 kDa
Observed band size: 17 kDa

Exposure time: 1 minute 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751685) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA751685_2.jpg Fig2: Western blot analysis of FGF1 on different lysates with Rabbit anti-FGF1 antibody (HA751685) at 1/5,000 dilution.

Lane 1: Neuro-2a cell lysate (30 µg/Lane)
Lane 2: Mouse kidney tissue lysate (30 µg/Lane)
Lane 3: Mouse heart tissue lysate (30 µg/Lane)
Lane 4: Rat heart tissue lysate (30 µg/Lane)

Predicted band size: 17 kDa
Observed band size: 16 kDa

Exposure time: 40 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751685) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA751685_3.jpg Fig3: Flow cytometric analysis of U-87 MG cells labeling FGF1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA751685, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA751685_4.jpg Fig4: Flow cytometric analysis of Neuro-2a cells labeling FGF1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA751685, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.