Thymidine Kinase 1 / TK1 Recombinant Rabbit Monoclonal Antibody [PSH18-97]
cat.: HA751687
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH18-97 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Thymidine Kinase 1 / TK1. |
| Positive control: | MOLT-4 cell lysate, U-2 OS cell lysate, SW480 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, MCF7 cell lysate, human tonsil tissue, human breast cancer tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:1,000 |
| Uniprot #: | SwissProt: P04183 Human |
| Alternative names: | cytosolic KITH_HUMAN Thymidine kinase 1 Thymidine kinase 1 soluble Thymidine kinase 1 soluble isoform Thymidine kinase Thymidine kinase cytosolic TK 1 TK1 |
Images
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Fig1:
Western blot analysis of Thymidine Kinase 1 / TK1 on different lysates with Rabbit anti-Thymidine Kinase 1 / TK1 antibody (HA751687) at 1/5,000 dilution. Lane 1: MOLT-4 cell lysate Lane 2: U-2 OS cell lysate Lane 3: SW480 cell lysate Lane 4: Jurkat cell lysate Lane 5: HEK-293 cell lysate Lane 6: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751687) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Thymidine Kinase 1 / TK1 antibody (HA751687) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751687) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Thymidine Kinase 1 / TK1 antibody (HA751687) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751687) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.