FGF23 Recombinant Rabbit Monoclonal Antibody [PSH19-00]
cat.: HA751690
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH19-00 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human FGF23 aa 1-251. |
| Positive control: | HeLa cell lysate, HT-29 cell lysate, PC-3 cell lysate, RAW264.7 cell lysate, Mouse thymus tissue lysate, Rat thymus tissue lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q9GZV9 Human | Q9EPC2 Mouse | Q8VI82 Rat |
| Alternative names: | ADHR FGF-23 Fgf23 FGF23_HUMAN FGFN Fibroblast growth factor 23 Fibroblast growth factor 23 C-terminal peptide Fibroblast growth factor 23 precursor HPDR2 HYPF Phosphatonin PHPTC Tumor derived hypophosphatemia inducing factor Tumor-derived hypophosphatemia-inducing factor |
Images
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Fig1:
Western blot analysis of FGF23 on different lysates with Rabbit anti-FGF23 antibody (HA751690) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HT-29 cell lysate (20 µg/Lane) Lane 3: PC-3 cell lysate (20 µg/Lane) Lane 4: RAW264.7 cell lysate (20 µg/Lane) Lane 5: Mouse thymus tissue lysate (30 µg/Lane) Lane 6: Mouse brain tissue lysate (negative) (30 µg/Lane) Lane 7: Rat thymus tissue lysate (30 µg/Lane) Predicted band size: 28 kDa Observed band size: 35 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751690) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.