Agrin Recombinant Rabbit Monoclonal Antibody [PSH19-50]
cat.: HA751714
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH19-50 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 217 kDa |
| Isotype: | IgG |
| Positive control: | HepG2 cell lysate, HT-29 cell lysate, U-2 OS cell lysate, NRK cell lysate, C6 cell lysate, HT-29. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IF-Cell FC |
1:5,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: O00468 Human | P25304 Rat |
| Alternative names: | AGRIN Agrin C-terminal 22 kDa fragment Agrin proteoglycan AGRIN_HUMAN Agrn C22 C90 FLJ45064 OTTHUMP00000044043 |
Images
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Fig1:
Western blot analysis of Agrin on different lysates with Rabbit anti-Agrin antibody (HA751714) at 1/5,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HT-29 cell lysate Lane 3: U-2 OS cell lysate Lane 4: NRK cell lysate Lane 5: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 217 kDa Observed band size: 22/90/110/250-300 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751714) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HT-29 cells labeling Agrin with Rabbit anti-Agrin antibody (HA751714) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Agrin antibody (HA751714) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of HT-29 cells labeling Agrin. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751714, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.