alpha Tubulin (acetyl K40) Recombinant Rabbit Monoclonal Antibody [PSH19-53]
cat.: HA751717
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH19-53 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human alpha Tubulin aa 11-60 / 451 (acetyl K40). |
| Positive control: | HeLa treated with 500ng/mL TSA for 4 hours cell lysate, NIH/3T3 treated with 400nM TSA for 18 hours cell lysate, C6 treated with 1μM TSA for 18 hours cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, HeLa cells treated with 500ng/mL TSA for 4 hours. |
| Subcellular location: | Cytoplasm, cytoskeleton. |
| Recommended Dilutions:
WB IP IF-Cell |
1:5,000 1-2μg/sample 1:5,000 |
| Uniprot #: | SwissProt: P68366 Human | P68368 Mouse | Q5XIF6 Rat |
| Alternative names: | Alpha tubulin ubiquitous Alpha-tubulin ubiquitous K alpha 1 TBA1B_HUMAN TUBA1B Tubulin alpha 1B Tubulin alpha 1B chain Tubulin alpha ubiquitous Tubulin alpha ubiquitous chain Tubulin alpha-1B chain Tubulin alpha-ubiquitous chain Tubulin K alpha 1 Tubulin K-alpha-1 |
Images
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Fig1:
Western blot analysis of alpha Tubulin (acetyl K40) on different lysates with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA751717) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 500ng/mL TSA for 4 hours cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate (20 µg/Lane) Lane 5: C6 cell lysate Lane 6: C6 treated with 1μM TSA for 18 hours cell lysate (20 µg/Lane) Lane 7: Mouse brain tissue lysate (40 µg/Lane) Lane 8: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: Lane 1-6: 6 seconds; Lane 7-8: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751717) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
alpha Tubulin (acetyl K40) was immunoprecipitated from 0.2 mg HeLa treated with 1μM TSA for 18 hours cell lysate with HA751717 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751717 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa treated with 1μM TSA for 18 hours cell lysate (input) Lane 2: HA751717 IP in HeLa treated with 1μM TSA for 18 hours cell lysate Lane 3: Rabbit IgG instead of HA751717 in HeLa treated with 1μM TSA for 18 hours cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 6 seconds; ECL: K1801 |
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Fig3:
Immunocytochemistry analysis of HeLa cells untreated / treated with 500ng/mL TSA for 4 hours labeling alpha Tubulin (acetyl K40) with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA751717) at 1/5,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin (acetyl K40) antibody (HA751717) at 1/5,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.