Dicer Recombinant Rabbit Monoclonal Antibody [PSH19-54]
cat.: HA751718
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH19-54 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 219 kDa |
| Isotype: | IgG |
| Positive control: | HeLa cell lysate, Daudi cell lysate, MCF7 cell lysate, 293T cell lysate, PC-12 cell lysate. |
| Subcellular location: | Cytoplasm, perinuclear region. |
| Recommended Dilutions:
WB IP |
1:1,000-1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q9UPY3 Human | Q8R418 Mouse Entrez Gene: 299284 Rat |
| Alternative names: | DCR DCR1 Dicer 1 ribonuclease III Dicer 1 ribonuclease type III Dicer DICER_HUMAN DICER1 Double-strand-specific ribonuclease Endoribonuclease Dicer Helicase MOI Helicase with RNase motif HERNA KIAA0928 MNG1 RMSE2 |
Images
|
Fig1:
Western blot analysis of Dicer on different lysates with Rabbit anti-Dicer antibody (HA751718) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: MCF7 cell lysate Lane 4: 293T cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 219 kDa Observed band size: 250 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751718) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Dicer on NIH/3T3 cell lysates with Rabbit anti-Dicer antibody (HA751718) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 219 kDa Observed band size: 250 kDa Exposure time: 1 minute 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751718) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Dicer was immunoprecipitated from 0.2 mg HeLa cell lysate with HA751718 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751718 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA751718 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA751718 in HeLa cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 2 minutes 6 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.