CD3 Recombinant Rabbit Monoclonal Antibody [PSH19-70]
cat.: HA751725
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC, mIHC |
| Clonality: | Monoclonal |
| Clone number: | PSH19-70 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Positive control: | MOLT-4 cell lysate, Jurkat cell lysate, mouse spleen tissue, rat spleen tissue, MOLT-4, mouse spleen. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC mIHC |
1:2,000 1:8,000 1:500 1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P07766 Human | P22646 Mouse Entrez Gene: 315609 Rat |
| Alternative names: | 4930549J05Rik A430104F18Rik AW552088 Cd247 CD247 antigen CD247 antigen, zeta subunit CD247 molecule CD3 CD3 antigen, delta subunit CD3 delta CD3 epsilon CD3 eta CD3 gamma CD3 molecule delta polypeptide CD3 molecule, epsilon polypeptide CD3 molecule, gamma polypeptide CD3 zeta CD3-DELTA CD3d CD3D antigen delta polypeptide CD3d antigen, delta polypeptide (TiT3 complex) CD3d molecule delta CD3d molecule delta CD3 TCR complex CD3d molecule, delta (CD3-TCR complex) CD3D_HUMAN CD3E CD3e antigen CD3E antigen epsilon polypeptide CD3e antigen, epsilon polypeptide (TiT3 complex) CD3e molecule epsilon CD3e molecule epsilon CD3 TCR complex CD3e molecule, epsilon (CD3-TCR complex) CD3epsilon CD3G CD3g antigen CD3G antigen gamma polypeptide CD3g antigen, gamma polypeptide (TiT3 complex) CD3g molecule gamma CD3g molecule gamma CD3 TCR complex CD3g molecule, gamma (CD3-TCR complex) CD3H CD3Q CD3Z CD3zeta Ctg3 FLJ...... |
Images
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Fig1:
Western blot analysis of CD3 on different lysates with Rabbit anti-CD3 antibody (HA751725) at 1/2,000 dilution. Lane 1: MOLT-4 cell lysate Lane 2: Raji cell lysate (negative) Lane 3: Jurkat cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 23 kDa Observed band size: 20 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751725) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD3 antibody (HA751725) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751725) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CD3 antibody (HA751725) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751725) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of MOLT-4 cells labeling CD3 with Rabbit anti-CD3 antibody (HA751725) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD3 antibody (HA751725) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Immunocytochemistry analysis of mouse spleen cells labeling CD3 with Rabbit anti-CD3 antibody (HA751725) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD3 antibody (HA751725) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig6:
Flow cytometric analysis of MOLT-4 cells labeling CD3. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751725, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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