Recoverin Recombinant Rabbit Monoclonal Antibody [PSH19-99]
cat.: HA751740
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH19-99 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Recoverin aa 1-202. |
| Positive control: | Y79 cell lysate, Mouse eyeball tissue lysate, Rat eyeball tissue lysate, mouse eyeball tissue, rat eyeball tissue. |
| Subcellular location: | Photoreceptor inner segment, Cell projection, cilium, photoreceptor outer segment, Photoreceptor outer segment membrane, Perikaryon. |
| Recommended Dilutions:
WB IHC-Fr IHC-P IF-Tissue IP |
1:5,000 1:500 1:5,000 1:200-1:500 1-2μg/sample |
| Uniprot #: | SwissProt: P35243 Human | P34057 Mouse Entrez Gene: 140936 Rat |
| Alternative names: | 23 kDa photoreceptor cell-specific protein Cancer associated retinopathy protein Cancer-associated retinopathy protein CAR CAR protein p26 Protein CAR RCV1 RCVRN RECO_HUMAN Recoverin S-modulin |
Images
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Fig1:
Western blot analysis of Recoverin on different lysates with Rabbit anti-Recoverin antibody (HA751740) at 1/5,000 dilution. Lane 1: Y79 cell lysate (20 µg/Lane) Lane 2: Raji cell lysate (negative) (20 µg/Lane) Lane 3: Mouse eyeball tissue lysate (40 µg/Lane) Lane 4: Mouse liver tissue lysate (negative) (40 µg/Lane) Lane 5: Rat eyeball tissue lysate (40 µg/Lane) Lane 6: Rat liver tissue lysate (negative) (40 µg/Lane) Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751740) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Application: IHC-Fr Species: Mouse Site: eyeball Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse eyeball tissue with Rabbit anti-Recoverin antibody (HA751740) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751740) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-Recoverin antibody (HA751740) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751740) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat eyeball tissue with Rabbit anti-Recoverin antibody (HA751740) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751740) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat liver tissue (negative) with Rabbit anti-Recoverin antibody (HA751740) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751740) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Application: IF-Tissue Species: Mouse Site: eyeball Sample: Paraffin-embedded section Antibody concentration: 1/200 |
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Fig8:
Application: IF-Tissue Species: Rat Site: eyeball Sample: Paraffin-embedded section Antibody concentration: 1/500 |
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Fig9:
Recoverin was immunoprecipitated from 0.2 mg Y79 cell lysate with HA751740 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751740 at 1/5,000 dilution. Alpaca anti-Rabbit IgG Fc secondary antibody (HA1031) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Y79 cell lysate (input) Lane 2: HA751740 IP in Y79 cell lysate Lane 3: Rabbit IgG instead of HA751740 in Y79 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 2 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.