Complement Factor D Recombinant Rabbit Monoclonal Antibody [PSH20-03]
cat.: HA751744
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH20-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Complement Factor D aa 21-259. |
| Positive control: | Mouse plasma tissue lysate, Mouse serum tissue lysate, mouse lung tissue lysates. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IP |
1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: P03953 Mouse |
| Alternative names: | Adipsin Adipsin/complement factor D Adn C3 convertase activator CFAD_HUMAN CFD Complement factor D complement factor D preproprotein D component of complement DF FactorD PFD Properdin factor D |
Images
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Fig1:
Western blot analysis of Complement Factor D on different lysates with Rabbit anti-Complement Factor D antibody (HA751744) at 1/5,000 dilution. Lane 1: Mouse plasma tissue lysate Lane 2: Mouse serum tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 28 kDa Observed band size: 45-50 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751744) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Complement Factor D on mouse lung tissue lysates with Rabbit anti-Complement Factor D antibody (HA751744) at 1/5,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 28 kDa Observed band size: 45-50 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751744) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Complement Factor D was immunoprecipitated from 0.2 mg mouse serum lysate with HA751744 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751744 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: mouse serum lysate (input) Lane 2: HA751744 IP in mouse serum lysate Lane 3: Rabbit IgG instead of HA751744 in mouse serum lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 46 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.