HGF Recombinant Rabbit Monoclonal Antibody [PSH20-29]
cat.: HA751745
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: IHC-P
Clonality: Monoclonal
Clone number: PSH20-29
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 83 kDa
Isotype: IgG
Immunogen: Recombinant protein within human HGF aa 1-494.
Positive control: Human placenta tissue.
Subcellular location: Extracellular region, extracellular space, membrane, platelet alpha granule lumen.
Recommended Dilutions:
  IHC-P

1:200
Uniprot #: SwissProt: P14210 Human
Alternative names: DFNB39 F TCF Fibroblast derived tumor cytotoxic factor Hepatocyte growth factor (hepapoietin A; scatter factor) Hepatocyte growth factor Hepatocyte growth factor beta chain Hepatocyte growth factor precursor Hepatopoietin A Hepatopoietin-A Hgf HGF_HUMAN HGFB HPTA Lung fibroblast derived mitogen OTTHUMP00000161349 OTTHUMP00000206710 OTTHUMP00000206711 OTTHUMP00000206712 OTTHUMP00000206713 OTTHUMP00000206730 Scatter factor SF
Images
HA751745_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-HGF antibody (HA751745) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751745) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.