eNOS Recombinant Rabbit Monoclonal Antibody [PSH20-32]
cat.: HA751748
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH20-32 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 133 kDa |
| Isotype: | IgG |
| Positive control: | EA.hy926 cell lysate, Mouse kidney tissue lysate. |
| Subcellular location: | Cell membrane, Membrane, caveola, Cytoplasm, cytoskeleton, Golgi apparatus. |
| Recommended Dilutions:
WB IP |
1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: P29474 Human | P70313 Mouse |
| Alternative names: | cNOS Constitutive NOS EC NOS EC-NOS ecNOS Endothelial nitric oxidase synthase Endothelial nitric oxide synthase Endothelial nitric oxide synthase 3 Endothelial NOS eNOS Nitric oxide synthase 3 (endothelial cell) Nitric oxide synthase 3 Nitric oxide synthase 3 endothelial cell Nitric oxide synthase endothelial Nitric oxide synthase, endothelial NOS 3 NOS III NOS type III NOS3 NOS3_HUMAN NOSIII |
Images
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Fig1:
Western blot analysis of eNOS on different lysates with Rabbit anti-eNOS antibody (HA751748) at 1/2,000 dilution. Lane 1: HeLa cell lysate (negative) (20 µg/Lane) Lane 2: EA.hy926 cell lysate (20 µg/Lane) Lane 3: RAW264.7 cell lysate (negative) (20 µg/Lane) Lane 4: Mouse kidney tissue lysate (no heat) (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 133 kDa Observed band size: 133 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751748) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
eNOS was immunoprecipitated from 0.2 mg EA.hy926 cell lysate with HA751748 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751748 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: EA.hy926 cell lysate (input) Lane 2: HA751748 IP in EA.hy926 cell lysate Lane 3: Rabbit IgG instead of HA751748 in EA.hy926 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 2 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.