KMT1E / SETDB1 Recombinant Rabbit Monoclonal Antibody [PSH20-40]
cat.: HA751756
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH20-40 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 143 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SETDB1 aa 1-400. |
| Positive control: | Jurkat cell lysate, T-47D cell lysate, 293T cell lysate, HeLa cell lysate, K-562 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, F9 cell lysate, PC-12 cell lysate, human colon cancer tissue, human colon tissue, human testis tissue, mouse colon tissue, mouse testis tissue, rat colon tissue, rat testis tissue. |
| Subcellular location: | Nucleus, Cytoplasm, Chromosome. |
| Recommended Dilutions:
WB IHC-P IP |
1:5,000 1:200-1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q15047 Human | O88974 Mouse Entrez Gene: 689883 Rat |
| Alternative names: | AU022152 EC 2.1.1.43 ERG-associated protein with SET domain ESET H3 K9 HMTase 4 H3-K9-HMTase 4 H3-K9-HMTase4 Histone H3 K9 methyltransferase 4 Histone H3-K9 methyltransferase 4 Histone-lysine N-methyltransferase SETDB1 KG1T KIAA0067 KMT1E Lysine N-methyltransferase 1E MGC90670 mKIAA0067 SET domain bifurcated 1 Set domain protein bifurcated 1 SETB1_HUMAN Setdb1 |
Images
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Fig1:
Western blot analysis of KMT1E / SETDB1 on different lysates with Rabbit anti-KMT1E / SETDB1 antibody (HA751756) at 1/5,000 dilution. Lane 1: Jurkat cell lysate Lane 2: T-47D cell lysate Lane 3: 293T cell lysate Lane 4: HeLa cell lysate Lane 5: K-562 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: F9 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 143 kDa Observed band size: 180 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751756) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-KMT1E / SETDB1 antibody (HA751756) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751756) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-KMT1E / SETDB1 antibody (HA751756) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751756) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KMT1E / SETDB1 antibody (HA751756) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751756) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-KMT1E / SETDB1 antibody (HA751756) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751756) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-KMT1E / SETDB1 antibody (HA751756) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751756) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-KMT1E / SETDB1 antibody (HA751756) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751756) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-KMT1E / SETDB1 antibody (HA751756) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751756) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9:
KMT1E / SETDB1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA751756 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751756 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA751756 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA751756 in Jurkat cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 30 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.