OGDH Recombinant Rabbit Monoclonal Antibody [PSH20-61]
cat.: HA751762
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH20-61 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 116 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse OGDH aa 524-1,023. |
| Positive control: | LNCaP cell lysate, HeLa cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, THP-1 cell lysate, THP-1 treated with 50ng/mL PMA for 72 hours add 1μg/mL LPS for last 24 hours cell lysate, Mouse heart tissue lysate, Mouse kidney tissue lysate, Rat heart tissue lysate, Rat kidney tissue lysate, human heart tissue, human kidney tissue, mouse heart tissue, mouse kidney tissue, rat heart tissue, rat kidney tissue, HeLa, NIH/3T3, C6. |
| Subcellular location: | Mitochondrion, Nucleus. |
| Recommended Dilutions:
WB IHC-Fr IHC-P IF-Cell |
1:5,000-1:200,000 1:500 1:1,000 1:100 |
| Uniprot #: | SwissProt: Q02218 Human | Q60597 Mouse | Q5XI78 Rat |
| Alternative names: | 2-oxoglutarate dehydrogenase complex component E1 EC:1.2.4.2 E1o; HsOGDH; OGDC-E1; OGDH-E1 2-oxoglutarate dehydrogenase, mitochondrial Alpha-ketoglutarate dehydrogenase (Alpha-KGDH-E1) Thiamine diphosphate (ThDP)-dependent 2-oxoglutarate dehydrogenase OGDH |
Images
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Fig1:
Western blot analysis of OGDH on different lysates with Rabbit anti-OGDH antibody (HA751762) at 1/5,000 dilution. Lane 1: LNCaP cell lysate Lane 2: HeLa cell lysate Lane 3: MCF7 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 116 kDa Observed band size: 116 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751762) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of OGDH on different lysates with Rabbit anti-OGDH antibody (HA751762) at 1/5,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 50ng/mL PMA for 72 hours add 1μg/mL LPS for last 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 116 kDa Observed band size: 116 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751762) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of OGDH on different lysates with Rabbit anti-OGDH antibody (HA751762) at 1/200,000 dilution. Lane 1: Mouse heart tissue lysate (10 µg/Lane) Lane 2: Mouse kidney tissue lysate (20 µg/Lane) Lane 3: Rat heart tissue lysate (10 µg/Lane) Lane 4: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 116 kDa Observed band size: 100 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751762) at 1/200,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Application: IHC-Fr Species: Mouse Site: kidney Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-OGDH antibody (HA751762) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751762) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-OGDH antibody (HA751762) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751762) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-OGDH antibody (HA751762) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751762) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-OGDH antibody (HA751762) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751762) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-OGDH antibody (HA751762) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751762) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig10:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-OGDH antibody (HA751762) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751762) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig11:
Immunocytochemistry analysis of HeLa cells labeling OGDH with Rabbit anti-OGDH antibody (HA751762) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-OGDH antibody (HA751762) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI. |
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Fig12:
Immunocytochemistry analysis of NIH/3T3 cells labeling OGDH with Rabbit anti-OGDH antibody (HA751762) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-OGDH antibody (HA751762) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI. |
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Fig13:
Immunocytochemistry analysis of C6 cells labeling OGDH with Rabbit anti-OGDH antibody (HA751762) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-OGDH antibody (HA751762) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI. |
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