TIMP4 Recombinant Rabbit Monoclonal Antibody [PSH20-91]
cat.: HA751773
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH20-91 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TIMP4 aa 1-224. |
| Positive control: | HeLa cell lysate, U-87 MG cell lysate, HT-29 cell lysate, MDA-MB-231 cell lysate, HEK-293 cell lysate, BxPC-3 cell lysate, Raji cell lysate, COS-1 cell lysate, Mouse brain tissue lysate, Mouse heart tissue lysate, Rat brain tissue lysate, Rat heart tissue lysate, U-87 MG. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB FC |
1:5,000 1:1,000 |
| Uniprot #: | SwissProt: Q99727 Human | Q9JHB3 Mouse | P81556 Rat |
| Alternative names: | Metalloproteinase inhibitor 4 TIMP 4 TIMP metallopeptidase inhibitor 4 TIMP-4 Timp4 TIMP4_HUMAN Tissue inhibitor of metalloproteinase 4 Tissue inhibitor of metalloproteinases 4 |
Images
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Fig1:
Western blot analysis of TIMP4 on different lysates with Rabbit anti-TIMP4 antibody (HA751773) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: U-87 MG cell lysate (20 µg/Lane) Lane 3: HT-29 cell lysate (20 µg/Lane) Lane 4: MDA-MB-231 cell lysate (20 µg/Lane) Lane 5: HEK-293 cell lysate (20 µg/Lane) Lane 6: BxPC-3 cell lysate (20 µg/Lane) Lane 7: Raji cell lysate (20 µg/Lane) Lane 8: COS-1 cell lysate (20 µg/Lane) Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751773) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of TIMP4 on different lysates with Rabbit anti-TIMP4 antibody (HA751773) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Mouse heart tissue lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Rat heart tissue lysate (20 µg/Lane) Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751773) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Flow cytometric analysis of U-87 MG cells labeling TIMP4. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751773, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.