ICAM3 Recombinant Rabbit Monoclonal Antibody [PSH20-98]
cat.: HA751780
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH20-98 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ICAM3 aa 1-485. |
| Positive control: | U-937 cell lysate, Jurkat cell lysate, Ramos cell lysate, THP-1 cell lysate, HL-60 cell lysate, Jurkat, human tonsil tissue, human spleen tissue, human colon tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:5,000 1:50 1:200-1:1,000 |
| Uniprot #: | SwissProt: P32942 Human |
| Alternative names: | CD50 CDW 50 CDw50 ICAM 3 ICAM-3 ICAM-R ICAM3 ICAM3_HUMAN ICAMR Intercellular adhesion molecule 3 |
Images
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Fig1:
Western blot analysis of ICAM3 on different lysates with Rabbit anti-ICAM3 antibody (HA751780) at 1/5,000 dilution. Lane 1: U-937 cell lysate Lane 2: Jurkat cell lysate Lane 3: Ramos cell lysate Lane 4: THP-1 cell lysate Lane 5: HL-60 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 100-150 kDa Exposure time: 17 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751780) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of ICAM3 on different lysates with Rabbit anti-ICAM3 antibody (HA751780) at 1/5,000 dilution. Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 2: Jurkat treated with PNGseF cell lysate Lane 3: U-937 (Human acute monocytic leukemia cells) cell lysate Lane 4: U-937 treated with PNGseF cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 17 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA751780, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 60 kDa Observed band size: 100-150/60 kDa |
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Fig3:
Immunocytochemistry analysis of Jurkat cells labeling ICAM3 with Rabbit anti-ICAM3 antibody (HA751780) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ICAM3 antibody (HA751780) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-ICAM3 antibody (HA751780) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751780) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-ICAM3 antibody (HA751780) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751780) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ICAM3 antibody (HA751780) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751780) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.