LDL Receptor Recombinant Rabbit Monoclonal Antibody [PSH20-99]
cat.: HA751781
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH20-99 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 95 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human LDL Receptor aa 1-788. |
| Positive control: | HEK-293 cell lysate, Huh7 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, Huh7. |
| Subcellular location: | Cell membrane, Membrane, clathrin-coated pit, Golgi apparatus, Early endosome, Late endosome, Lysosome. |
| Recommended Dilutions:
WB IF-Cell |
1:5,000 1:200 |
| Uniprot #: | SwissProt: P01130 Human | P35951 Mouse | P35952 Rat |
| Alternative names: | FH FHC LDL R LDL receptor LDLCQ2 Ldlr LDLR_HUMAN Low Density Lipoprotein Receptor Low density lipoprotein receptor class A domain containing protein 3 Low density lipoprotein receptor familial hypercholesterolemia Low-density lipoprotein receptor |
Images
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Fig1:
Western blot analysis of LDL Receptor on different lysates with Rabbit anti-LDL Receptor antibody (HA751781) at 1/5,000 dilution. Lane 1: HEK-293 cell lysate (low expression) (20 µg/Lane) Lane 2: Huh7 cell lysate (20 µg/Lane) Lane 3: Mouse liver tissue lysate (30 µg/Lane) Lane 4: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 95 kDa Observed band size: 150 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751781) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Huh7 cells labeling LDL Receptor with Rabbit anti-LDL Receptor antibody (HA751781) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LDL Receptor antibody (HA751781) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.