Fas / CD95 Recombinant Rabbit Monoclonal Antibody [PSH21-00]
cat.: HA751782
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | IHC-Fr, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH21-00 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Fas / CD95 aa 1-169. |
| Positive control: | Mouse liver tissue, A20. |
| Subcellular location: | Cell membrane, Membrane raft. |
| Recommended Dilutions:
IHC-Fr IF-Cell FC |
1:500 1:100 1:1,000 |
| Uniprot #: | SwissProt: P25446 Mouse |
| Alternative names: | ALPS 1A ALPS1A APO 1 Apo 1 antigen APO 1 cell surface antigen Apo-1 antigen APO1 Apo1 antigen APO1 cell surface antigen Apoptosis antigen 1 Apoptosis mediating surface antigen FAS Apoptosis-mediating surface antigen FAS APT 1 APT1 CD 95 CD 95 antigen CD95 CD95 antigen Delta Fas Delta Fas/APO 1/CD95 Delta Fas/APO1/CD95 Fas (TNF receptor superfamily, member 6) FAS 1 FAS 827dupA Fas AMA Fas FAS Antigen Fas cell surface death receptor FAS1 FASLG receptor FASTM sFAS Surface antigen APO1 TNF receptor superfamily, member 6 TNFRSF 6 TNFRSF6 Tumor necrosis factor receptor superfamily member 6 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Immunocytochemistry analysis of A20 (positive) and L-929 (low expression) labeling Fas / CD95 with Rabbit anti-Fas / CD95 antibody (HA751782) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Fas / CD95 antibody (HA751782) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of L-929 (left, low expression) and A20 (right, positive) cells labeling Fas / CD95. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751782, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.