Fas / CD95 Recombinant Rabbit Monoclonal Antibody [PSH21-01]
cat.: HA751783
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-Fr, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH21-01 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Fas / CD95 aa 1-169. |
| Positive control: | 3T3-L1 cell lysate, L-929 cell lysate, CTLL-2 cell lysate, L-929, 3T3-L1, A20. |
| Subcellular location: | Cell membrane, Membrane raft. |
| Recommended Dilutions:
WB IHC-Fr FC IP |
1:2,000-1:5,000 1:500 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P25446 Mouse |
| Alternative names: | ALPS 1A ALPS1A APO 1 Apo 1 antigen APO 1 cell surface antigen Apo-1 antigen APO1 Apo1 antigen APO1 cell surface antigen Apoptosis antigen 1 Apoptosis mediating surface antigen FAS Apoptosis-mediating surface antigen FAS APT 1 APT1 CD 95 CD 95 antigen CD95 CD95 antigen Delta Fas Delta Fas/APO 1/CD95 Delta Fas/APO1/CD95 Fas (TNF receptor superfamily, member 6) FAS 1 FAS 827dupA Fas AMA Fas FAS Antigen Fas cell surface death receptor FAS1 FASLG receptor FASTM sFAS Surface antigen APO1 TNF receptor superfamily, member 6 TNFRSF 6 TNFRSF6 Tumor necrosis factor receptor superfamily member 6 |
Images
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Fig1:
Western blot analysis of Fas / CD95 on different lysates with Rabbit anti-Fas / CD95 antibody (HA751783) at 1/5,000 dilution. Lane 1: 3T3-L1 cell lysate Lane 2: L-929 cell lysate (low expression) Lane 3: CTLL-2 cell lysate (low expression) Lysates/proteins at 20 µg/Lane. Predicted band size: 37 kDa Observed band size: 40 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751783) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Fas / CD95 on A20 cell lysates with Rabbit anti-Fas / CD95 antibody (HA751783) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 37 kDa Observed band size: 40 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751783) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Application: IHC-Fr Species: Mouse Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig4:
Flow cytometric analysis of L-929 (left, low expression) and 3T3-L1 (right, positive) cells labeling Fas / CD95. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751783, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Flow cytometric analysis of A20 cells labeling Fas / CD95. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751783, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6:
Fas / CD95 was immunoprecipitated from 0.2 mg 3T3-L1 cell lysate with HA751783 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751783 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 3T3-L1 cell lysate (input) Lane 2: HA751783 IP in 3T3-L1 cell lysate Lane 3: Rabbit IgG instead of HA751783 in 3T3-L1 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 1 minute 2 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.