SSBP1 Recombinant Rabbit Monoclonal Antibody [PSH21-31]
cat.: HA751794
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH21-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SSBP1 aa 1-148. |
| Positive control: | A549 cell lysate, A375 cell lysate, NIH:OVCAR-3 cell lysate, 293T cell lysate, A431 cell lysate, MCF7 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, C6 cell lysate, Mouse liver tissue lysate, Mouse pancreas tissue lysate, Rat liver tissue lysate, Rat pancreas tissue lysate, human brain tissue, human pancreas tissue, rat brain tissue, rat pancreas tissue. |
| Subcellular location: | Mitochondrion, Mitochondrion matrix, mitochondrion nucleoid. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: Q04837 Human | Q9CYR0 Mouse | P28042 Rat |
| Alternative names: | Single-stranded DNA-binding protein, mitochondrial Mt SSB Mt-SSB MtSSB PWP1 interacting protein 17 PWP1-interacting protein 17 SENSOR OF SINGLE-STRANDED DNA COMPLEX Single stranded DNA binding protein 1 Single stranded DNA binding protein Single stranded DNA binding protein mitochondrial Single-stranded DNA-binding protein SOSS B1 SOSS COMPLEX SUBUNIT B1 SSBP 1 SSBP SSBP_HUMAN SSBP1 |
Images
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Fig1:
Western blot analysis of SSBP1 on different lysates with Rabbit anti-SSBP1 antibody (HA751794) at 1/5,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: A375 cell lysate (20 µg/Lane) Lane 3: NIH:OVCAR-3 cell lysate (20 µg/Lane) Lane 4: 293T cell lysate (20 µg/Lane) Lane 5: A431 cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: COS-1 cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: Neuro-2a cell lysate (20 µg/Lane) Lane 10: C6 cell lysate (20 µg/Lane) Lane 11: Mouse liver tissue lysate (40 µg/Lane) Lane 12: Mouse pancreas tissue lysate (40 µg/Lane) Lane 13: Rat liver tissue lysate (40 µg/Lane) Lane 14: Rat pancreas tissue lysate (40 µg/Lane) Predicted band size: 17 kDa Observed band size: 14 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751794) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SSBP1 antibody (HA751794) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751794) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-SSBP1 antibody (HA751794) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751794) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-SSBP1 antibody (HA751794) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751794) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-SSBP1 antibody (HA751794) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751794) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.